How to Reconstitute Xeomin: Step-by-Step Guide

Reconstituting Xeomin involves mixing its powder with sterile saline to create an injectable solution. Use 0.9% sodium chloride (non-preserved saline) and follow aseptic techniques. Typical dilution ratios range from 1-4 mL per 100-unit vial, depending on the desired concentration. Always discard unused solution after 24 hours.

What You'll Need

  • 1 vial of Xeomin powder (100 units)
  • Sterile 0.9% sodium chloride (saline) (non-preserved)
  • 1-5 mL syringe (with 21-30G needle for drawing saline)
  • Alcohol swabs
  • Sterile mixing vial or tray
  • Insulin syringe (for injection, if applicable)

Step-by-Step Reconstitution Process

  1. Prepare the workspace: Clean a flat surface with alcohol swabs and gather supplies.
  2. Draw saline: Attach a needle to a syringe and draw 1-4 mL of sterile saline (common ratios: 1 mL = 10 units/0.1 mL; 2.5 mL = 4 units/0.1 mL).
  3. Inject saline into Xeomin vial: Pierce the vial's rubber stopper and slowly add saline down the side to avoid foaming.
  4. Mix gently: Swirl the vial do not shake to dissolve the powder. Let it sit for 1-2 minutes if needed.
  5. Check for clarity: The solution should be clear and colorless. Discard if cloudy or discolored.
  6. Draw the dose: Use a new syringe to withdraw the required units. Replace the needle if injecting.
  7. Store properly: Refrigerate at 2-8°C (36-46°F) and use within 24 hours.

Reconstitution Ratios & Concentrations

Saline Volume (mL) Units per 0.1 mL Total Doses (0.1 mL Injections) Best For
1.0 10 units 10 High-precision areas (e.g., crow's feet, forehead)
2.0 5 units 20 Moderate dilution (versatile for most treatments)
4.0 2.5 units 40 Large areas or lower-dose injections (e.g., masseters)

Critical Tips for Safety & Effectiveness

  • Avoid foaming: Foam denatures the toxin. Inject saline slowly and avoid vigorous shaking.
  • Use non-preserved saline: Preservatives (e.g., benzyl alcohol) can degrade Xeomin.
  • Discard after 24 hours: Reconstituted Xeomin loses potency quickly, even if refrigerated.
  • Label the vial: Note the date/time of reconstitution and concentration.
  • Needle size matters: Use 30-32G needles for injections to minimize pain and bruising.

Common Mistakes to Avoid

  • Over-diluting: Too much saline reduces precision (e.g., 5 mL = 2 units/0.1 mL, which is hard to measure accurately).
  • Under-diluting: High concentrations (e.g., 0.5 mL) can cause over-treatment or diffusion.
  • Reusing needles: Always use a new needle for drawing and injecting to prevent contamination.
  • Freezing Xeomin: Freezing destroys the toxin-store only in the refrigerator.
  • Ignoring expiration: Unopened vials expire ~3 years from manufacture; reconstituted solution expires in 24 hours.