Mix tirzepatide 5mg with 1.5mL sterile water for injection to achieve 3.33mg/mL concentration.
To reconstitute tirzepatide 5mg, add 1.5mL of sterile water for injection to the vial containing the lyophilized powder. Gently swirl the vial until the powder dissolves completely. The resulting solution will have a concentration of 3.33mg/mL, providing 0.45mL per dose for a 1.5mg weekly injection. Store the reconstituted solution in the refrigerator and use within 21 days.
Step-by-step reconstitution process
- Wash hands thoroughly and clean the vial tops with alcohol swabs
- Draw 1.5mL of sterile water into a syringe
- Insert the needle into the tirzepatide vial and slowly inject the water
- Gently swirl the vial until all powder dissolves (do not shake)
- Inspect the solution for clarity and absence of particles
- Label the vial with date and time of reconstitution
Storage and handling guidelines
| Condition | Duration |
|---|---|
| Unreconstituted (refrigerated) | Until expiration date |
| Reconstituted (refrigerated) | 21 days maximum |
| Reconstituted (room temperature) | 8 hours maximum |
| Freezing | Not recommended |
Dosage calculation and administration
Each 5mg vial provides approximately 3.33 doses at the standard 1.5mg weekly dose. To administer: 1. Withdraw 0.45mL (1.5mg) using an insulin syringe 2. Inject subcutaneously in the abdomen, thigh, or upper arm 3. Rotate injection sites weekly 4. Administer on the same day each week, with or without food
Common mistakes to avoid
- Using incorrect diluent volume (should be 1.5mL, not 2.5mL)
- Shaking the vial vigorously, which can denature the peptide
- Using tap water or non-sterile diluents
- Storing reconstituted solution beyond 21 days
- Freezing the solution, which damages the medication